Project description
Following infection, bunyaviruses initiate a cascade of events that convert the host cell into a virion production line, historically termed a viral factory (VF), where transcription, translation, replication, and virion assembly drive infectivity and disease, yet key spatio-temporal and structural information about VF formation and host factor involvement remains incomplete or inaccurate. To address this, we aim to determine how the bunyavirus VF is built by identifying where and when functional stages of the viral life cycle occur and uncovering host factors involved in VF formation and activity. For this we use genome-wide, machine learning approaches, and correlative light and cryo-EM methods.