Project description
High-throughput biochemical assays are typically limited to in vitro systems or indirect measurements of molecular targets. Leveraging our lab’s catalog of repeat-binding factors, we developed a high-throughput screening platform to identify chemical modulators of dozens of transcription factors, many of which are established drivers of human cancers. Importantly, this technology enables a direct, live-cell readout of protein–DNA interactions. By preserving the chromatin architecture and post-translational regulatory states within the cellular milieu, this method recapitulates the complete biological context of transcription factor binding which is absent from traditional biochemical approaches. A patent is pending for this methodology.